Biological activities of endemic Prangos hulusii
Prangos species have been traditionally used to stop bleeding and as a wound and scar healer, emollient, antifungal, antihemorrhoidal, antioxidant in Turkey (1). P. hulusii S.G. Şenol, H. Yıldırım & Ö. Seçmen is one of the newly identified endemic species and no researches on it has been done yet (2).
This study was aimed to investigate cytotoxic, antioxidant and antibacterial capacity of aerial parts (AP) and roots (RP) of P. hulusii which is widely used in folk medicine. The AP and RP were extracted successively with petroleum ether, dichlorometan and methanol in Soxhlet apparatus (1). For antioxidant effect, inhibition of lipid peroxidation in liposomes, induced by Fe3+/ascorbate system, scavenging effect on DPPH· and superoxide radicals and reducing power were analysed. The antibacterial activity was investigated against Gram(+) and Gram(-) reference bacterias (S. aureus, E. coli, K. pneumonia, B. cereus, P. vulgaris) and yeast C. albicans by using standard microbroth dilution and disk diffusion methods. Also, the cytotoxic activities of the crude extracts were examined by LDH (Lactate dehydrogenase) and MTT (mitochondrial succinate dehydrogenase) assays in the rat kidney epithelial cell line.
As a result, AP extracts showed better antioxidant activity than RP extracts with the strongest potency for the methanol, followed by dichlorometan extracts. In the MTT test, the crude extracts were not cytotoxic at studied concentrations (0.5 – 0.125 mg crude extract/ml for AP, 0 – 0.2 mg crude extract/ml for RP), however, LDH test results showed that only petroleum ether extracts demonstrated low cytotoxicty. The antimicrobial activities of all extracts were observed against S. aureus, B. cereus and yeast.
 Razavi M.S., Zarrini G., Zahri S., Mohammadi S., Biological activity of Prangos uloptera DC. Roots, A Medicinal Plant from Iran, Nat Prod Res, 24,9,797 – 3,(2010)
 Şenol S.G., Yıldırım H., Seçmen Ö.S., Prangos hulusii sp.nov. from West Anatolia, Turkey. Nord J Bot,29,402 – 7(2011).