Planta Med 2013; 79 - PN103
DOI: 10.1055/s-0033-1352445

Antioxidative and neuroprotective activities of isocampneoside II on H2O2-induced oxidative injury in PC12 cells

C Si 1, G Xu 2, T Shen 3, J Jiang 2, X Ren 2
  • 1a: Tianjin Key Lab of Pulp & Paper, Tianjin University of Science &Technology, Tianjin 300457, China; b: Tianjin Key Lab of Marine Resources & Chemistry, Tianjin University of Science & Technology, Tianjin 300457, China
  • 2Tianjin Key Lab of Pulp & Paper, College of Materials Science & Chemical Engineering, Tianjin University of Science &Technology, Tianjin 300457, China
  • 3Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University, Huaian 223300, China

Oxidative stress has been considered as a major cause of cell damage in a variety of neurodegenerative disorders. One of the reasonable strategies for delaying the disease's progression is to prevent the reactive oxygen species (ROS) mediated cellular injury by dietary or pharmaceutical augmentation of free radical scavengers [1]. Isocampneoside II (ICD) is an active phenylethanoid glycoside compound isolated from Paulownia tomentosa var. tomentosa (Scrophulariaceae) [2]. The present study was designed to explore the free radical scavenging potential of ICD in different in vitro systems and its protective role in H2O2 induced oxidative stress and apoptotic death in cultured rat pheochromocytoma (PC12) cells. The results showed that ICD eliminated approximately 80.75% superoxide radical at the concentration of 0.1 mg/ml and inhibited metal chelating by 22.07% at 8 mg/ml. In addition, ICD showed a strong ability on reducing power and provided protection against oxidative protein damage induced by hydroxyl radicals. Pretreatment of PC12 cells with ICD prior to H2O2 exposure elevated the cell viability, enhanced activity of superoxide dismutase (SOD) and catalase, and decreased the levels of malondialdehyde (MDA) and intracellular ROS. Furthermore, ICD inhibited cell apoptosis and Bax/Bcl-2 ratio induced by H2O2. These findings suggested that ICD may be considered as a potential antioxidant agent and should encourage for further research in neurodegenerative diseases.

References:

[1] Hsu CL, et al. (2010). J Agric Food Chem 58: 2150 – 2156

[2] Si CL. et al. (2011) Holzforschung 62: 197 – 200.

Acknowledgements: This work was financed by National Natural Science Foundation of China (31170541 & 31000279), Program for New Century Excellent Talents in University (NCET-10 – 0951), Natural Science Foundation of Tianjin City (No. 13JCZDJC), and Foundation (201204) of Tianjin Key Lab of Marine Resources & Chemistry, Tianjin University of Science & Technology, China.