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DOI: 10.1055/s-0033-1352347
Anti-cholinesterase activity of 5-Theins recipes potentially used for Alzheimer's disease
Background: In Thai traditional medicine, brain hypo-function, which is similar to Alzheimer's disease (AD), is due to declining in wind-element, blood circulation and neurological function. Review of Traditional Medicine scripture about wind-element, most frequently prescribed plants were group of 5-Theins as Lepidium sativum L.(Ls), Nigella sativa L.(Ns), Cuminum cyminum L. (Cc), Foeniculum valgare L.(Fv), Anethum graveolens L.(Ag). Aims: To study acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities of the 5-Theins remedy and its ingredient. Method: 30 grams of each plant and the 5-Theins were separately extracted by 95% ethanol, evaporated and vacuum-dried at 45 °C. By Elman method, both enzymes activities were assessed. The constituents of each extract were determined by GC-MS. Results: All extracts at dose of 0.1 mg/ml exhibited higher BuChE inhibitory activity than that of AChE. The IC50 of BuChE inhibitory activity of Ls, 5-Theins(EtOH) and galantamine were 5.59,12.27 and 0.95 µg/ml. The constituents of each extract were shown in table.
sample |
AChuEI activity |
BuChuEI activity |
Main constituents determined by GC-MS |
|
% inh. (0.1 mg/ml) |
% inh. (0.1 mg/ml) |
IC50 (µg/ml) |
||
Ns |
26.16 (2.9) |
21.41 (1.2) |
> 200 |
eicosanoic acid, fatty acid |
Ls |
32.63 (2.9) |
91.45 (0.6) |
5.59 (0.6) |
cumialdehyde, apiol, isoanethol, thymoquinone |
Cc |
27.64 (1.2) |
41.35 (0.7) |
128.36 (5.3) |
cumialdehyde, eicosanoic acid |
Fv |
22.94 (0.6) |
18.86 (1.2) |
> 200 |
isoanethol, oleic acid ethyl ester, cumialdehyde |
Ag |
29.61(1.8) |
18.94 (1.2) |
> 200 |
diterpene derivative, L-carvone |
5-Theins (EtOH) |
29.5 (0.9) |
80.77 (1.0) |
12.27 (0.7) |
oleic acid ethyl ester, linoleic acid, apiol, cumialdehyde |
Galantamine |
93.68 (1.0) |
74.27 (0.9) |
0.95 (0.03) |
Conclusion: The combined recipe had synergistic effect on BuChE inhibitory activity more than single herb except Ls. These results revealed the potential used of Ls and the 5-Theins for AD treatment.
“This work was supported by research fund of Thammasat University.”