Planta Med 2013; 79 - PN3
DOI: 10.1055/s-0033-1352347

Anti-cholinesterase activity of 5-Theins recipes potentially used for Alzheimer's disease

P Akkapinya 1, C Sattaponpan 2, A Itharat 1
  • 1Applied Thai Traditional Medicinal Medicine, Faculty of Medicine, Thammasat University, Pathumthani, Thailand
  • 2Research Center, Faculty of Medicine, Thammasat University, Pathumthani, Thailand

Background: In Thai traditional medicine, brain hypo-function, which is similar to Alzheimer's disease (AD), is due to declining in wind-element, blood circulation and neurological function. Review of Traditional Medicine scripture about wind-element, most frequently prescribed plants were group of 5-Theins as Lepidium sativum L.(Ls), Nigella sativa L.(Ns), Cuminum cyminum L. (Cc), Foeniculum valgare L.(Fv), Anethum graveolens L.(Ag). Aims: To study acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities of the 5-Theins remedy and its ingredient. Method: 30 grams of each plant and the 5-Theins were separately extracted by 95% ethanol, evaporated and vacuum-dried at 45 °C. By Elman method, both enzymes activities were assessed. The constituents of each extract were determined by GC-MS. Results: All extracts at dose of 0.1 mg/ml exhibited higher BuChE inhibitory activity than that of AChE. The IC50 of BuChE inhibitory activity of Ls, 5-Theins(EtOH) and galantamine were 5.59,12.27 and 0.95 µg/ml. The constituents of each extract were shown in table.

sample

AChuEI activity

BuChuEI activity

Main constituents determined by GC-MS

% inh. (0.1 mg/ml)

% inh. (0.1 mg/ml)

IC50 (µg/ml)

Ns

26.16 (2.9)

21.41 (1.2)

> 200

eicosanoic acid, fatty acid

Ls

32.63 (2.9)

91.45 (0.6)

5.59 (0.6)

cumialdehyde, apiol, isoanethol, thymoquinone

Cc

27.64 (1.2)

41.35 (0.7)

128.36 (5.3)

cumialdehyde, eicosanoic acid

Fv

22.94 (0.6)

18.86 (1.2)

> 200

isoanethol, oleic acid ethyl ester, cumialdehyde

Ag

29.61(1.8)

18.94 (1.2)

> 200

diterpene derivative, L-carvone

5-Theins (EtOH)

29.5 (0.9)

80.77 (1.0)

12.27 (0.7)

oleic acid ethyl ester, linoleic acid, apiol, cumialdehyde

Galantamine

93.68 (1.0)

74.27 (0.9)

0.95 (0.03)

Conclusion: The combined recipe had synergistic effect on BuChE inhibitory activity more than single herb except Ls. These results revealed the potential used of Ls and the 5-Theins for AD treatment.

“This work was supported by research fund of Thammasat University.”