Planta Med 2013; 79 - PK39
DOI: 10.1055/s-0033-1352299

Chromatographic examination of Aremonia agrimonoides (L.) DC (Rosaceae)

S Pilipovic 1, A Uzunovic 1, A Elezovic 1
  • 1Agency for Medicinal Products and Medical Devices, Titova 9, 71000, Sarajevo, Bosnia and Herzegovina

Aremonia agrimonoides,(L.) DC (Rosaceae), is a native plant species in central Europe. As the continuation of previously performed research [1], this work represents a research by chromatographic dry herbal material. Plant materials were collected in Bosnia and Herzegovina during May 2011. Two HPTLC methods were performed. The first method was described by Harborne [2] and the second method was described by Lee and others [3]. The third method was two dimensional TLC on Cellulose MN300 plates as described by Harborn [2]. Spot detection was observed under UV light and by spraying with: vanillin/HCl reagent, sodium nitrite reagent, saturated KIO3 water solution, Folin-Ciocalteu reagent. In table 1. we present our results.

Tab. 1: HPTLC spots with vanillin/HCl reagent (red spots) and Folin-Ciocalteu reagent (grey spots)

Method

Part of herb

Rf (catechin standard Sigma Aldrich)

Rf

(Rf (procyandidin B1 Sigma Aldrich)

Rf

Rf

HPTLC method 1

Aremoniae herba

0.81

–

0.63

–

–

Aremoniae rhizoma cum radicibus

0.81

–

0.63

0.43

0.35

HPTLC method 2

Aremoniae herba

0.45

–

0.27

0.18

–

Aremoniae rhizoma cum radicibus

0.45

0.32

0.27

0.18

0.11

In TLC method with Cellulose MN300 plates after spraying with saturated KIO3 we observed a spot with brown color on start line (possible ellagitannins). In table 1. we present spots of catechins (react with Folin-Ciocalteu (phenolics) and vanillin/HCl reagent (catechins). In rhizome and herb we find catechin, dimers and trimers of catechin. on Rf 0.32 according to Lea it is possible to be procyanidin B5. Aremonia agrimonoides, (L.) DC (Rosaceae) possesses anti-inflammatory activity (1) and can be possible source of catechins.

References:

[1] Pilipovic S, Mulabegovic N, Mornjakovic Z, Uzunovic A, Elezovic A, Hadzidedic S: Anti-inflammatory activity of ointments with dry extracts of rhizome and herb of Aremonia agrimonoides, (L.) DC (Rosaceae). Planta Med 2011; 77: 1430

[2] Harborne JB. Phenolic compounds In: Phytochemical methods: A guide to modern techniques of plant analysis. 3 rd ed. Harborne JB. London, Chapman & Hall. 1998; 40 – 106.

[3] Lea AGH, Arnold GM. Phenolics of ciders – bitterness and astringency. J. Sci. Food Agric. 1978; 29: 478 – 483