Planta Med 2013; 79 - PK27
DOI: 10.1055/s-0033-1352287

Effect of seed pretreatment by H2O2 and magnetic field on the sensitivity of Silybum marianum L. to salinity sea water

MM Migahid 1, RM Abd Elraouf 1, LM Bidak 2, AW Amin 2
  • 1Faculty of Education biology and Geology department Alexandria university Alexandria Egypt, 21526
  • 2Faculty of Science Botany department Alexandria university Alexandria Egypt, 21526

The effects of seed pretreatment by H2O2 and magnetic field (MF) on the impacts of sea water concentration were tested using Silybum marianum (S. marianum) at the vegetative stage. Some soaked S. marianum seeds were subjected to 0.18 T MF for various duration (0, 10, 20 and 30 min) and other seeds were soaked in different concentration of H2O2 (0, 80,160 and 240µM) for 8h. After germination the seeds were sowed in homogeneous soil and irrigated with tap water and 10% sea water concentration. Some effects of both MF and H2O2 pretreatment under sea water treatment were investigated. H2O2 pretreatment increased growth and development under control condition more than under sea water stress. On the other hand, our results provided evidence that seed H2O2 and MF-pretreatment increased the tolerance of S. marianum to salinity. In conclusion, our study demonstrated that sea water stress caused highly significant reduction in the growth parameters and stimulation in proline and phenolic compounds. Pretreatment seeds of S. marianum with H2O2 and magnetic field in different duration may alleviate the oxidative damage leading to improvements in physiological attributes for the plant growth under sea water stress.

Keywords: Silybum marianum; Magnetic field; Physiological responses; H2O2, Sea water