Polyphenolic fingerprints of different organs of Rosa hybrida cv. 'Jardin de Granville'

Rosa hybrida cultivar 'Jardin de Grandville', a delicate clear pink flower, was selected by Parfums Christian Dior to enter in some luxury cosmetic formulations. This cultivar has been selected not only for its beauty but also for its resistance to diseases. In order to characterize the phytochemical content of this plant, responsible of its singularity, different plant organs are investigated. Analyses are focused on the polyphenol family, widely described in the literature for their large diversity of functions in the plant (coloration, antioxidant activities, defense molecules against diseases and pathogens...). The investigation of these polyphenolic compounds in Rosa hybrida cv. 'Jardin de Grandville' is of a great interest, considering its high level of selection.

The phytochemical content of 16 plant organs is evaluated: winter woods, shoots, early buds, buds before flowering, flowers (first flowering period, spring), four flower parts analyzed separately (petals, sepals, receptacles and stamens), leaves, summer woods, summer shoots, buds before flowering, flowers (second flowering period, summer), roots and fruits. Reversed phase UHPLC technique coupled with DAD and ESI-UHR-Q-TOF mass spectrometry was used to manage the analyses.

Combining all the information collected (absorbance maxima, accurate mass and molecular formulae) and thanks to comparison with reference standards, several phenolic compounds have been characterized like tannins derived from quinic, gallic and ellagic acid, quercetin derivatives and kaempferol derivatives.

Statistical analyses (PCA, HAC and ANOVA) are applied to this data set to point out similarities or differences between the different parts of the plant. A clear separation between vegetative and reproductive organs is obtained. Thus, compounds like kaempferol derivatives are representative of the reproductive organs whereas catechin derivatives are more abundant in the vegetative parts.