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DOI: 10.1055/s-0033-1352233
Separation of phenylethanoid glycosides from two Lantana species by High-Speed Countercurrent Chromatography
The genus Lantana (Verbenaceae) comprises approximately 150 species, distributed in tropical and subtropical regions. Lantana fucata Lindl. is a small shrub used in the Brazilian traditional medicine as a carminative, anti-inflammatory, and to treat colds and bronchitis. In a previous work from our group, the alcoholic extract from this plant and Fucatoside C, isolated from it, showed significant in vitro antiinflammatory activity (Julião et al., 2009). Lantana trifolia L. is used in Brazilian folk medicine for respiratory disorders and as sedative. Its antiinflammatory and analgesic activities were also demonstrated by us (Julião et al., 2010). In the present study, n-butanol extracts from leaves of L. fucata and L. trifolia were fractionated by HSCCC as a new strategy to isolate the active compounds for biological investigations. CCC is a technique based on liquid-liquid extractions and uses no solid stationary phase. The choice of solvent system was performed by test-tube partition test. The solvent system chosen was ethyl acetate/n-butanol/water (1.0:0.2:1.0, v/v) for both n-butanol extracts. The isocratic elutions (normal phase) were performed on HSCCC equipped with a multi-layer coil, conditions as follows: column 80 mL, 1.6 mm id, flow rate 2.0 mL/min (4.0 mL/tube), 60 tubes, 850 rpm, temperature 26 °C, loop injection 5.0 mL, wash off = 80 mL. Stationary phase retention (Sf) was 75%. Two phenylethanoids – Numioside A (23 mg, 77.4%) and Fucatoside C (31.9 mg, 77%) were isolated from L. fucata, and two from L. trifolia: Verbascoside (29.5 mg, 80.5%) and Apiosylverbascoside (39.3 mg, 81.8%). Purities were determined by HPLC. All substances were isolated in one-step separation from circa 400 mg of each extract, in a much more efficient and costless process than the other techniques used in the previous works. Support: CAPES and CNPq.
References:
[1] Julião LS; et al., J. Nat. Prod., 72, 1424 – 1428, 2009;
[2] Julião LS; et al. Phytochemistry, 71, 294 – 300, 2010.