Planta Med 2013; 79 - PI111
DOI: 10.1055/s-0033-1352200

Chemical constituents from Neonectria coronata YMJ94043006

C Ya-Chih 1, H Che-Jen 2, J Yu-Min 3, L Tzong-Huei 4
  • 1College of Pharmacy, Taipei Medical University, Taipei, Taiwan
  • 2School of Respiratory Therapy, Taipei Medical University, Taipei, Taiwan
  • 3Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
  • 4Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan

Four novel 10-membered macrolides, namely neonectrilactones A–D (1–4), together with four cylindrols 5–8 and three ascochlorins 9–11, were isolated from the ethyl acetate extracts of the fermented broths of Neonectria coronata YMJ94043006. The structures of 1–11 were elucidated on the basis of spectroscopic data analyses. The relative configurations of 1–4 were deduced mainly by 1H-NMR and NOESY, and compared with the literatures. Compounds 5-11 were subjected to growth inhibitory activity against A549 lung cancer line, and among the compounds tested, the chlorine-containing cylindrol analogues 9–11 exhibited potent activities with GI50 values of 1.2, 1.1 and 8.2µM, respectively, while 5–8 exhibited no activity even at a concentration higher than 30µM. We thus suggested that the chlorine would play a crucial role for the cytotoxicity of 9–11.