Planta Med 2013; 79 - PI83
DOI: 10.1055/s-0033-1352172

Pure isolation of biologically active compounds and proteins from different plants by adsorptive bubble separation

H Parlar 1, P Yilmaz 2, A Spangenberg 3
  • 1Technical University of Munich-Weihenstephan D-85354 Freising-Germany
  • 2Technical University of Munich-Weihenstephan D-85354 Freising-Germany
  • 3Technical University of Munich-Weihenstephan D-85354 Freising -Germany

Developing biologically active products from medial herbs is of interest to the pharmaceutical industries. For their isolation, methods such as solvent extraction or supercritical fluid extraction are employed. However these methods tend to burden the ecosystem by using organic solvents extensively or demand considerable attention and, hence greater investiment and costs. During the extractions, fat and chlorophyll are co-extracted and, thereafter have to be separated from the active substance by chromatographic methods. Alternative methods of interest are the so- called "Adsorptive Bubble Separation" and "Tweezing Adsorptive Bubble Separation1). Both methods are effective, especially at low concentrations of substances. Furthermore, they use inert gases, which make them mild methods for extracting substances that are sensitive to oxidation. As surfactants, saponine, gelatine and albumine at different pH can be used. These substances can be eliminated after the foam fractionation. During the last ten years, a great number of biologically active compounds such as gingeroles, catechines etc, enzymes such as laccases etc, and hormones for example insulin could be isolated by this way in pure form 2)

By applying tweezing-adsorptive bubble separation, Laccase C and Horseradish Peroxidase could be enriched 10 – 14-fold respectively, without significant loss of enzmatic activities3) Also, the enzymes MMP9 and Carboxypeptidase A could be separated with recoveries higher than 85%. The method is based on chelation of enzymes active center with a surface- active chelator such as N-(2-Acetamido)iminodiacetic acid coupled with an octyl- or nonyl- unit. The formed complexes are surface active and can be transferred easly to the foam phase.

References:

[1] M. Backleh-Sohrt, P. Ekici, G. Leupold, and H. Parlar. J. Nat. Prod. 68,1386(2005)

[2] A. Nicolai, A. Friess and H. Parlar. J. Sep. Sci.31,2310(2008)

[3] BM. Gerken, A. Nicolai, D. Zorn, RG. Berger and H. Parlar. Anal. Chem77,6113(2005)