Planta Med 2013; 79 - PI12
DOI: 10.1055/s-0033-1352102

Lipase inhibitory activity and phytochemical studies of Polygonum sericeum

M Orgilkhatan 1, M Toshihiro 2, K Kyoko 2, E Selenge 2, Y Fumihiko 2, J Batkhuu 1
  • 1School of Biology and Biotechnology, National University of Mongolia, P.O.B-617, Ulaanbaatar-46A, Mongolia
  • 2Department of Pharmacognosy, Tohoku Pharmaceutical University, 4 – 4-1 Komatsushima, Aoba-ku, Sendai 981 – 8558, Japan

More than 120 Mongolian plant species were screened for their in vitro Lipase inhibitory activity by BALB-DTNB method [1]. Among them, the stems of Polygonum sericeum showed significant antilipase activity. On the other hand, no reports have been published on chemistry and biological activity of this plant. P. sericeum Pall. Ex Georgi (Polygonaceae Juss) is an annual herbaceous plant, distributed in Khentei, Khangai, Mongol Daurian regions of Mongolia [2]. Stems of P. sericeum were extracted with 80% acetone. After filtration and evaporation in vacuo, the crude extract was suspended in water and then successively partitioned with chloroform and n-butanol. The n-butanol fraction exhibited the greatest lipase inhibitory activity with 70% at 3 mg/ml concentration. The bioactive fraction was subjected further to ODS column and HPLC, and afforded two new compounds (1, 2, Figure), together with four known compounds, such as quercetin (3), quercetin-3-O-β-D-glucuronide (4), quercetin-3-O-β-D-glucuronide-6″-methyl ester (5) and quercetin-3-O-α-arabinopyranoside (6). All isolates are reported for the first time in P. sericeum by our work. Structures of the two new compounds were determined on the basis of their physico-chemical properties as well as spectroscopic analyses including 1H, 13C, 2D NMR and MS.

Fig. 1

Acknowledgements: This study was partially supported by Honda Foundation, Japan.

References:

[1] I. Furukawa et al (1982) Clinical chemistry 28/1, 110 – 113.

[2] V.I. Grubov (1982). Key to the vascular plants of Mongolia (with an atlas) Nauka publishing, Leningrad, USSR.