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DOI: 10.1055/s-0033-1352091
Five triterpenoidal saponins from the butanol fraction of Balanites aegyptiaca Del
The butanol fraction of the 70% ethanolic extract of Balanites aegyptiaca fruit was fractionated using vacuum liquid chromatography (VLC RP-18 column). A gradient elution was performed using a mixture of methanol/water, yielding 5 sub-fractions monitored by analytical high performance liquid chromatography (HPLC). The sub-fraction eluted with 75% methanol was subjected to a preparative HPLC where five triterpenoidal saponins were isolated for the first time from Balanites aegyptiaca fruit. The isolated compounds were identified as: compound 1, 26-O-β-D-glucopyranosyl-(25R)-furost-5-ene-3,22,26-triol 3-O-[α-L-rhamnopyranosyl-(1→3)- β-D-glucopyranosyl-(1→2)- α-L-rhamnopyranosyl-(1→4)] β-D-glucopyranoside; compound 2, 26-O-β-D-glucopyranosyl-(25R)-furost-5-ene-3,22,26-triol 3-O-[ β-D-glucopyranosyl-(1→2)- α-L-rhamnopyranosyl-(1→4)] β-D-glucopyranoside; compound 3, 26-O-β-D-glucopyranosyl-(25R)-furost-5,20-diene-3,22,26-triol 3-O-[α-L-rhamnopyranosyl-(1→3)- β-D-glucopyranosyl-(1→2)- α-L-rhamnopyranosyl-(1→4)] β-D-glucopyranoside; compound 4, 26-O-β-D-glucopyranosyl-(25S)-furost-5,20-diene-3,22,26-triol 3-O-[ β-D-glucopyranosyl-(1→2)- α-L-rhamnopyranosyl-(1→4)] β-D-glucopyranoside; compound 5, 26-O-β-D-glucopyranosyl-(25R)-furost-5,20-diene-3,22,26-triol 3-O-[ β-D-glucopyranosyl-(1→2)- α-L-rhamnopyranosyl-(1→4)] β-D-glucopyranoside, by spectroscopical analysis [MS,1D and 2D NMR (HSQC, HMBC, DQF-COSY, HSQC-TOCSY)].
Acknowledgement: This work was carried out in frame of an FP7 project, Marie Curie Actions, PIRSES-GA-2008 – 230816. Project title is "Natural antidiabetic and anti-hypertensive drugs (NAAN)" and Prof. Rudolf Bauer is the project coordinator