Skin formulation containg Arrabidaea chica extract for wound-healing

Arrabideae chica (Bignoniaceae) popularly known as Carajiru, is a climbing plant widely distributed in South American tropical forest. Leaves of this plant are popularly use in skin diseases. (Devia et al. 2002). Wounding has a tremendous impact in healthcare economy; especially chronic wounds represent a major health burden. Recent investigations have shown that plant-derived secondary compounds can serve as new lead compounds for improvement of wound healing. Studies conducted at CPQBA-Unicamp showed that the crude A. chica extract has antiulcerogenic and healing action. The cutaneous ulcer lesion index was evaluated on animal experimental model with Wistar rats. The ulcers were assessed daily by measuring the contraction area and calculation of the percentage decrease (Jorge et.al. 2008). After 5 days of treatment the animals in the negative control groups (saline) or vehicle (Natrosol, Carbopol, cream W/O and cream O/W) were stressed presenting redness, exudate and pus, whereas animals treated with A. chica extract formulation had a more docile behavior and without pus. After 10 days the formulation evaluated in in vivo wound healing experimental models demonstrated 70 to 80% decrease of the ulcerated skin area compared with 37% reduction from the control group. The hidroxiproline content in wound tissue treated with different formulation was observed for the Natrosol ^TM gel formulation containing 0,093 µg.mL^-1 of crude extract when compared to other control (0,019 µg.mL^-1) and vehicle (0,028 µg.mL^-1). Histopathological study was showed that well organized fibers. Quality control of the crude extract was accessed by monitoring carajurin (6,50%w/w) content and luteolin (0,50%w/w) content by HPLC with DAD detector.