Planta Med 2013; 79 - PC9
DOI: 10.1055/s-0033-1352003

Evaluation of Caesalpinia peltophoroides in skin keratinocytes and dermal fibroblasts

JC Mello 1, FG Bueno 1, A Hensel 2, M Lechtenberg 2, F Petereit 2
  • 1Universidade Estadual de Maringá, Department of Pharmacy, BR-87020 – 900, Maringá, Brazil
  • 2University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, D-48149, Münster, Germany

Wound healing is a complex process involving several biological events, and a delay in this process may cause economic and social problems. Herbal compounds are assuming an important role in tissue repair1 and have been used as wound healing agents2. Therefore, the aim of this study was to evaluate the cell viability of keratinocytes (HaCat) and fibroblasts (NHDF) in vitro after treatment with C. peltophoroides crude extract (CE), using the MTT method.

C. peltophoroides barks were collected at UEM, Brazil, and extracted using 50% ethanol by turbo-extraction (CE). CE was partitioned with ethyl-acetate (EAF) and water. EAF was fractionated by column chromatography on Sephadex LH-20 using 50% ethanol (FA), 100% ethanol (FB) and 70% acetone (FC). FC was fractionated on MCI-Gel® CHP-20P column by gradient (methanol:water) to yield FC1 – 4. Fractions were monitored by analytical TLC and by HaCat and NHDF, using untreated control (UC-medium) and positive control (PC-5% FCS). The mitochondrial activity was evaluated by MTT test3. The statistical analyses were performed using Statistica® 8.0 (ANOVA) with P< 0.05 as the significance criteria.

An increased cell viability after treatment with CE and FC was observed in both cell cultures with a high activity at 25 µg/mL. The viability in case of HaCat treated with FC1 is higher compared to FC at 25 µg/mL. However, the NHDF viability increased after 10 µg/mL (Fig. 1).

It was observed that CE of C. peltophoroides stimulated in vitro cell viability of HaCat and NHDF.

Fig. 1: Cell Viability (%) of keratinocytes and fibroblasts in: crude extract, FA, FB, FC and FC1 – 4 (1 – 100 µg/mL) (*P< 0.05).

Acknowledgements: Fundação Araucária, CNPQ, DAAD.

References:

[1] Sehn, E., Hernandes, L., Franco, S.L., Gonçalves, C.C.M., Baesso, M.L. 2009. Analytica Chimica Acta, 115 – 120.

[2] Agyare, C., Lechtenberg, M., Deters, A., Petereit, F., Hensel, A. 2011. Phytomedicine, 617 – 624.

[3] Mosmann, T. 1983. Journal of Immunological Methods, 55 – 63.