Planta Med 2013; 79 - PB25
DOI: 10.1055/s-0033-1351970

Anti-allergic effect of a Korean folk medicine, KOTMIN5, in vitro and in vivo

S Kim 1, K Jeong 1, H Park 1, J Lee 2, H Lee 1, E Lee 1
  • 1Korea Promotion Institute for Traditional Medicine Industry, Research and Development Division, Gyeongsan 712 – 210, South Korea
  • 2Myongji University, Center for Nutraceutical and Pharmaceutical Materials, Yongin 449 – 728, South Korea

A Korean folk medicine, Biyeom Tang (KOTMIN5), composed of Xanthii fructus, Trichosanthis semen, Angelicae dahuricae radix, and Menthae herba, had been used in traditional medicinal clinic for the treatment of inflammatory diseases such as allergic rhinitis. However, the anti-allergic properties of KOTMIN5 and its molecular mechanisms have not been investigated. In the present study, the ethanol extract of KOTMIN5 was investigated for anti-allergic properties in bone-marrow derived mast cells (BMMC) and in vivo model. KOTMIN5 strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 17.6 mg/ml. In addition, the generation of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) was inhibited in BMMC in a concentration-dependent manner with IC50 values of 33.8 mg/ml and 24.3 mg/ml, respectively. Furthermore, KOTMIN5 reduced compound48/80-induced systemic anaphylactic shock and IgE-mediated passive cutaneous anaphylaxis in mice. These results suggested that KOTMIN5 might be useful in regulating allergic reactions.