Planta Med 2013; 79 - PA17
DOI: 10.1055/s-0033-1351921

Profiling the antiplasmodial polyphenolic fraction of Anogeissus leiocarpus leaves by LC/PDA/ESI-MS/MS

HA Ali 1, TJ Schmidt 2, SA Khalid 3
  • 1National Center for Research, Commission for Biotechnology and Genetic Engineering, Khartoum, Sudan
  • 2University of Münster, Institute of Pharmaceutical Biology and Phytochemistry (IPBP), Corrensstraße 48, D-48149 Münster, Germany
  • 3University of Science & Technology, Faculty of Pharmacy, P.O. Box 447, Omdurman, Sudan University of Khartoum, Faculty of Pharmacy, Department of Pharmacognosy, P.O. Box 1996 Khartoum, Sudan

Leaves decoctions of Anogeissus leiocarpus (Combretaceae) have long been used in the African traditional medicine to treat malaria, trypanosomiasis, dysentery and helminthiasis.1, 2 As part of our on-going activity within the “Research Network Natural Products against Neglected Diseases” (ResNet NPND) we subjected the polyphenolic ethyl acetate fraction which is associated with the antiplasmodial activity of A. leiocarpus leaves to chemical profiling with LC/PDA/ESI-MS/MS.

The 80% methanolic extract of leaves of A. leiocarpus was sequentially fractionated with petroleum ether, chloroform and ethyl acetate. The aforementioned fractions were tested against chloroquine sensitive Plasmodium falciparum NF54 with chromogenic assay involves the biological reduction by viable cells of the tetrazolium compound 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolium (MTS) at two different concentrations 10 µg/ml and 5 µg/ml. The ethyl acetate fraction significantly inhibited the malaria parasite (99%) at 10 µg/ml with no sign of toxicity.

RP-HPLC-DAD coupled with tandem mass spectrometry performed on the bioactive ethyl acetate fraction led to the identification of six derivatives of ellagic acid, mainly methyl ether and its glycosides besides four quercetin conjugates and a kaempferol glycoside as well as two stilbenes, namely E-viniferin and methoxy E-viniferin which were not previously reported from this genus.

References:

[1] Neuwinger, H.D. 2000. Press Stuttgart, Germany: 46.

[2] Okpekon, T.; Yolou, S.; Gleye, C.; Roblot, F.; Loiseau, P.; Bories, C.; Grellier, P.; Frappier F.; Laurens, A. and Hocquemiller, R., 2004. Journal of Ethnopharmacology, 90: 91 – 97.

[3] Adigun, J.O.; Amupitan, J.O. and Kelly, D.R., 2000. Bull. Chem. Soc. Ethiopia, 14(2): 169 – 174.