PEG2 inhibitory compounds from Dioscorea membranacea roots

A Itharat; S Ruangnoo; P Thongdeeying

doi:10.1055/s-0033-1351918

Planta Medica, Inhaltsverzeichnis

PEG2 inhibitory compounds from Dioscorea membranacea roots

A Itharat ¹, S Ruangnoo ², P Thongdeeying ²

¹Center of Excellence in Thai Traditional Medicine, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Klongluang Pathumtani, 12120, Thailand
²Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Klongluang Pathumtani, 12120, Thailand

Abstract

The roots of Dioscorea membrancea have been used as an antiinflammatory drug in Thai traditional medicine [1]. Bioassay-guided fractionation was used to isolate the anti-inflammatory ingredients by testing PEG2 inhibitory effect on RAW264.7 macrophages [2]. Dioscorearide B (1), dioscoreanone (2) and two dihydrophenantrene compounds, 2,4 dimethoxy-5,6-dihydroxy-9,10-dihydrophenanthrene (3) and 5-hydroxy-2,4,6-trimethoxy-9,10-dihydrophenanthrene (4), were isolated. 4 showed the highest PEG2 inhibitory effect, followed by 1 and3 (IC₅₀= 7.65, 20.83 and 28.56µM, respectively). Dioscoreanone had no PEG2 inhibitory effect. Indomethacin as a positive control showed inhibitory effect on PEG2 release with an IC₅₀ value of 2.8µM.

References:

[1] Pongboonrod, S (1976) Medicinal plants of Thailand. Kasem Bundit, Bangkok, Thailand pp180.

[2] Tewtrakul S and Itharat A (2007)J ethnopharmacol, 105,312 – 315.