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Exp Clin Endocrinol Diabetes 2013; 121(10): 635-642
DOI: 10.1055/s-0033-1351331
Article
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Effect of Aminoguanidine Intervention on Neutrophils in Diabetes Inflammatory Cells Wound Healing

M. Tian   #
1   Shanghai Burns Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Peoples’ Republic of China
,
C. Qing   #
1   Shanghai Burns Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Peoples’ Republic of China
,
Y. Niu
1   Shanghai Burns Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Peoples’ Republic of China
,
J. Dong
1   Shanghai Burns Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Peoples’ Republic of China
,
X. Cao
1   Shanghai Burns Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Peoples’ Republic of China
,
F. Song
1   Shanghai Burns Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Peoples’ Republic of China
,
X. Ji
1   Shanghai Burns Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Peoples’ Republic of China
,
S. Lu
1   Shanghai Burns Institute, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, Peoples’ Republic of China
› Author Affiliations
Further Information

Publication History

received 26 April 2013
first decision 02 July 2013

accepted 17 July 2013

Publication Date:
03 September 2013 (online)

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Abstract

To explore aminoguanidine (AG) effect on neutrophil functions and associated signal transduction way in diabetic rats wound healing. Sprague-Dawley (SD) rats were divided into 3 groups, Group A (control+burns), Group B (diabetes+burns), Group C (diabetes+burns+AG). Wound skin tissue was harvested at 6h, 24h and 36h after trauma, and then immunohistochemistry was used to detect AGE (advanced glycation end products) contents and RAGE (receptor of AGE) expression. Western blotting was applied to detect RAGE and NF-κB. Oxidative stress changes were detected by colorimetry. Inflammatory cytokines were determined by ELIASA and cell apoptosis by TUNEL. Pathological changes were analyzed by hematoxylin-eosin (HE) staining. In the wound tissue of Group C, compared to that in Group B, AGE content, RAGE expression level, NF-κB level declined, and MPO (myeloperoxidase) decreased at 36h; TNFα, IL-8, H2O2, GSH-Px (Glutathione peroxidse), and MDA (malondialdehyde) levels increased; dense post-traumatic inflammation belt formed obviously. AG for prophylactic use can promote the migration and respiratory burst of neutrophils markedly, and help to restore the functions of neutrophil; and the abnormal secretion of inflammation cytokines can be corrected partly. Blocking AGE deposition and promoting microenvironment were effective ways for diabetic wound healing.

Key words

aminoguanidine - neutrophils - diabetic wound healing

# Co-first author: These authors contributed equally to this work.


 

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