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Planta Med 2013; 79(14): 1362-1369
DOI: 10.1055/s-0033-1350646
Natural Product Chemistry
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Cardenolides and Bufadienolide Glycosides from Kalanchoe tubiflora and Evaluation of Cytotoxicity

Hui-Chi Huang
1   Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan
,
Ming-Kuem Lin
1   Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan
,
Hsin-Ling Yang
2   Department of Nutrition, China Medical University, Taichung, Taiwan
,
You-Cheng Hseu
3   Department of Cosmeceutics and Graduate Institute of Cosmeceutics, China Medical University, Taichung, Taiwan
,
Chih-Chuang Liaw
4   Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung, Taiwan
,
Yen-Hsueh Tseng
5   Department of Forestry, National Chung Hsing University, Taichung, Taiwan
,
Minoru Tsuzuki
6   Nihon Pharmaceutical University, Saitama, Japan
,
Yueh-Hsiung Kuo
1   Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan
7   Tsuzuki Institute for Traditional Medicine, College of Pharmacy, China Medical University, Taichung, Taiwan
› Author Affiliations
Further Information

Publication History

received 21 January 2013
revised 25 March 2013

accepted 25 June 2013

Publication Date:
22 July 2013 (online)

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Abstract

Two new cardenolides, kalantubolide A (1) and kalantubolide B (2), and two bufadienolide glycosides, kalantuboside A (3) and kalantuboside B (4), as well as eleven known compounds were isolated and characterized from the EtOH extract of Kalanchoe tubiflora. The structures of compounds were assigned based on 1D and 2D NMR spectroscopic analyses including HMQC, HMBC, and NOESY. Biological evaluation indicated that cardenolides (1–2) and bufadienolide glycosides (3–7) showed strong cytotoxicity against four human tumor cell lines (A549, Cal-27, A2058, and HL-60) with IC50 values ranging from 0.01 µM to 10.66 µM. Cardenolides (1–2) also displayed significant cytotoxicity toward HL-60 tumor cell line. In addition, compounds 3, 4, 5, 6, and 7 blocked the cell cycle in the G2/M-phase and induced apoptosis in HL-60 cells.

Key words

Chinese herb - cardenolides - bufadienolide glycosides - Crassulaceae - Kalanchoe tubiflora - apoptosis

Supporting Information

 

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