Preparative-Scale Isolation of Resveratrol Metabolites from Human Urine After Oral Intake of a Resveratrol-Containing Dietary Supplement

Major resveratrol metabolites encountered in human urine after oral administration of trans-resveratrol (t-RES) includes t-RES sulfates, glucuronides and conjugates of dihydroresveratrol. A method for preparative isolation of major resveratrol metabolites from human urine after oral intake of a t-RES-containing dietary supplement was developed in order to test and explore the bioactivity of resveratrol metabolites in vitro and in preclinical trials. The urine was pre-treated by using solid-phase extraction to give a brown oily residue, which was separated using a combination of different chromatographic methods resulting in the isolation of five t-RES metabolites (M1-M5).