Planta Med 2013; 79 - PP4
DOI: 10.1055/s-0033-1348803

Evaluation of Carajurin Content Arrabidaea Chica Using Different Solvents for Extraction

IMO Sousa 1, RAF Rodrigues 1, NCA Queiroz 1, MP Jorge 1, L Servat 1, MN Eberlin 1, MA Foglio 1
  • 1CPQBA- State University of Campinas, P.O. Box 6171, 13083 – 970 Campinas-SP, Brazil

Arrabidaea chica is a liana native to South America, commonly known as “Crajiru”. Leaves of A. chica are used in folk medicine as antidiarrheal and antianemic agents, and are also indicated to treat intestinal colic, leukemia, albuminuria, and skin infections (Jorge et al., 2008). The chemical constituents of a red dye present in the leaves of A. chica were examined and some desoxyanthocyanidins were identified, the most important of which are carajurin and carajurone Zorn et al., 2001; Devia et al., 2002). The aim of this study was to determinate the best experimental conditions (solvent water, ethanol/water; chloroform, ethanol/water and citric acid 0,3%) for the extraction of Carajurin (6,7-dihydroxy-5,4-dimethoxy-flavylium) and luteolin and others glycosylated flavonoids, which are probably responsible for A.chica's biological activity. The results obtained make it possible to recommend optimal procedural guidelines for different compounds present in A.chica. Determined by HPLC-DAD (Phenomenex Gemini C-18 column (4,6 mm x 250 mm i.d., 3 µm), flow rate 1 mL/min, mobile phase methanol: aqueous phosphoric acid (pH 2.00) and ESI-MS with direct infusion.The extraction with ethanol/water citric acid was able to extract carajurin (6.5%(w/w)) and luteolin (0.5% (w/w), obtaining a yield of 16% (w/w) for crude extract.