Planta Med 2013; 79 - PN79
DOI: 10.1055/s-0033-1348760

Proanthocyanidins from Whole Spenceria ramalana Plants and Their Effects on AGE Formation in Vitro and Vessel Dilation in Larval Zebrafish in Vivo

IS Lee 1, SY Yu 1, SH Jung 1, YM Lee 1, YR Lee 1, JH Kim 2, H Sun 3 JS Kim 1*
  • 1KM-Based Herbal Drug Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 305 – 811, Korea
  • 2Department of Life Science, Gachon University, Seongnam, Gyeonggi-do 461 – 701, Korea
  • 3Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650204, China

Three new A-type proanthocyanidins (1 – 3), ent-epiafzelechin-(2α→O→7, 4α→8)-ent-afzelechin 3'-O-β-D-glycopyranoside (1), ent-epiafzelechin-(2α→O→7, 4α→8)-ent-epiafzelechin-(2α→O→7, 4α→8)-ent-afzelechin (2), and ent-epiafzelechin-(2α→O→7, 4α→8)-ent-epicatechin-(2α→O→7, 4α→8)-ent-afzelechin (3), were isolated from the whole plant of Spenceria ramalana, together with three known compounds (4 – 6). The inhibitory effects of isolated compounds (1 – 6) on the formation of AGE were examined in vitro; compounds 3 and 6 showed the strongest inhibition, with IC50 values of 17.4 ± 0.9 and 14.1 ± 2.8 µM, respectively. The effects of these isolates on the dilation of hyaloid-retinal vessels induced by high glucose (HG) in larval zebrafish were also investigated. Compound 3 reduced the dilation of HG-induced hyaloid-retinal vessels most effectively.