Planta Med 2013; 79 - PN18
DOI: 10.1055/s-0033-1348699

Anti-Metalloproteinase-9 Activity of Orally Panduratin A Isolated from Kaempferia Pandurata Roxb. on Experimental Gingival Inflammation in the Rat

Yanti 1, JK Hwang 2
  • 1Faculty of Biotechnology, Atma Jaya Catholic University, Jalan Jenderal Sudirman 51, Jakarta 12930, Indonesia
  • 2Department of Biotechnology, Yonsei University, 262-Seongsanno, Seodaemun-gu, Seoul 120 – 749, Korea

Periodontotherapy using natural oral care functional foods has been a focus of research investigation. Here, we investigated the efficacy of orally panduratin A, a chalcone compound isolated from Kaempferia pandurata Roxb., on inhibition of matrix metalloproteinase-9 (MMP-9)-dependent gingival inflammation in rats by performing histochemistry, imunohistochemistry, zymography, Western blot, and RT-PCR analysis. Panduratin A was isolated from the ethanolic extract of K. pandurata using silica gel column chromatography, RP-18 column chromatography and prep-HPLC, and further identified by 1H-NMR, 13C-NMR, HSQC, HMBC and FAB-MS instruments. H&E and IHC stainings confirmed that orally panduratin A (25 and 100 mg/kg) effectively repaired gingival tissue morphology in rats and significantly suppressed the expression of MMP-9 secretion, protein, and mRNA. In line with these data, orally panduratin A also abolished urokinase-type plasminogen activator (uPA) secretion, protein, and mRNA in gingival rat inflammation. Therefore, panduratin A may be used as a potent nutraceutical for controlling periodontal inflammation