Planta Med 2013; 79 - PF3
DOI: 10.1055/s-0033-1348586

Identification of SQAP-Binding Proteins from a T7 Phage Display Screen

J Izaguirre-Carbonell 1, H Murata 1, K Ohta 1, T Kusayanagi 1, S Tsukuda 1, K Iwabata 1, Y Kanai 1, S Kamisuki 1, K Sakaguchi 1, F Sugawara 1
  • 1Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 – 8510, Japan

Sulfoquynovosylacylpropanediol (SQAP, below figure) was chemically designed from SQMG isolated from sea urchins as a result of natural products research. SQAP performed synergistically inhibition of angiogenesis at low doses in a combination therapy with ionizing radiation. Four SQAP-binding candidate proteins were identified by a T7 phage display screen as follows: sterol carrier protein 2 (SCP-2), multifunctional enzyme type 2 (MFE-2), proteasomal ubiquitin receptor (ADRM1) and UV excision repair protein (HR23B). Two independent experiments were used to verify the interactions between SQAP and the observed candidate peptides. In a parallel research, downregulation of histone deacetylase 1 (HDAC1) was also observed under SQAP treatment.