Planta Med 2013; 79 - P74
DOI: 10.1055/s-0033-1336516

Stimulatory Effect on Osteoblast Differentiation of Compounds from Cissus quadrangularis L

T Pathomwichaiwat 1, P Ochareon 2, Z Ali 3, IA Khan 3, 4, S Prathanturarug 1
  • 1Department of Pharmaceutical Botany, Faculty of Pharmacy and
  • 2Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand
  • 3National Center for Natural Products Research and
  • 4Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA

Cissus quadrangularis L. (CQ), a medicinal plant, is widely used in traditional medicine in Southeast Asia [1]. A number of studies reported health benefits of this plant while many CQ products have been sold as dietary supplements [2]. However, the chemical standardization of CQ pharmacologically active compound has never been indicated. Our previous investigation found that the CQ hexane extract possessed antiosteoporotic effect in ovariectomized mice model [3]. The hexane extract was further isolated using bioassay-guided purification by bone cell culture technique. Five of the 31 sub-fractions stimulated the differentiation process of MC3T3-E1 osteoblast-like cells. The pharmacologically active compounds contained in these sub-fractions were isolated and elucidated. These active compounds will be useful for standardization of both raw material and CQ products. Acknowledgements: This project is financially supported by the Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0201/2549) and the Chao Phraya Abhaibhubejhr Hospital Foundation. References: [1] Williamson EM (2002) Major herbs of Ayuraveda. Churchill Livingstone. London. [2] Stohs SJ, Ray SD (2012) Phytother Res. doi: 10.1002/ptr.4846. [3] Pathomwichaiwat T, et al. (2012) Asian Biomed 6: 377 – 384.