Angiotensin Converting Enzyme (ACE) Inhibition Activity of Standardized Extract of the Ocimum sanctum L. Leaves and its Fractions

In vitro Angiotensin Converting Enzyme (ACE) inhibition activity was determined with standardized extract of the Ocimum sanctum L. leaves and its fractions. The ACE inhibition activity and antioxidant activity were investigated by a UV method [1]. In the ACE inhibition method, the synthetic substrate hippuryl-L-histidyl-L-leucine was allowed to react with a test sample containing ACE, to produce hippuric acid and ACE inhibition activity was calculated. Different concentrations of methanol extract of O. sanctum and eugenol were subjected to HPLC analysis using the mobile phase (methanol: water: acetic acid; 60%: 40%: 1%). O. sanctum oil, eugenol and ethyl acetate fractions showed the maximum ACE inhibition activity in a concentration-dependent manner with IC₅₀ value of 32.11 ± 3.6, 42.16 ± 2.7 and 56.83 ± 2.8 µg/mL, respectively. O. sanctum extract/fractions, eugenol and O. sanctum oil inhibited ACE in a concentration-dependent manner and are the most responsible for the antihypertensive action.

Acknowledgements: The authors wish to express their gratitude to the Council of Scientific and Industrial Research, Government of India for financial support through EMR project grant [F.No.-60(0084)/08/EMR-II/2009]. References: [1] Chaudhary SK, Mukherjee PK, et al. (2012) Orient Pharm Exp Med, DOI 10.1007/s13596 – 012 – 0076 – 0