Drug Res (Stuttg) 2013; 63(02): 90-93
DOI: 10.1055/s-0032-1333227
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Effect of the Treatment with Alpha-Macroglobulin on the Development of Experimental Pancreatitis

V. E. Di Loreto
1  Bone Biology Laboratory, School of Medicine Rosario National University, Rosario, Argentina
,
S. M. Roma
1  Bone Biology Laboratory, School of Medicine Rosario National University, Rosario, Argentina
,
A. Rigalli
1  Bone Biology Laboratory, School of Medicine Rosario National University, Rosario, Argentina
› Author Affiliations
Further Information

Publication History

received 01 August 2012

accepted 11 December 2012

Publication Date:
17 January 2013 (online)

Abstract

Alpha-macroglobulins are proteinase inhibitors. Monofluorophosphate increases alpha-macroglobulin levels in plasma, inducing a higher survival rate and lower pancreatic damage in rats with pancreatitis. The aim of this study was to evaluate the effect of alpha-macroglobulin on the development of pancreatitis. Pancreatitis was surgically induced in Sprague-Dawley rats divided into groups of 16 rats each and ­subjected to the following intravenous treatments for 3 days: Controls: pancreatitis without treatment, Enriched plasma: pancreatitis+­alpha-macroglobulin-enriched plasma, Normal plasma: pancreatitis+plasma with normal levels of alpha-macroglobulin, Saline Solution: pancreatitis+saline solution, Purified alpha-macroglobulin: pancreatitis+purified alpha-macroglobulin. After 14 days pancreatic damage was assessed using a score that measures: edema, fibrin, neutrophils, mononuclear leukocytes, necrosis, vascular congestion, thrombosis, hemorrhage and fibrosis. Pancreatic damage decreased and the percentage of animals with pancreatitis was lower in enriched-plasma and purified alpha-macroglobulin groups. We conclude that the intravenous administration of alpha-macroglobulins causes a reduction in the histological damage produced by pancreatitis.