Broad Spectrum Antibacterial Activity of a Mixture of Isothiocyanates from Nasturtium (Tropaeoli majoris herba) and Horseradish (Armoraciae rusticanae radix)

A. Conrad; D. Biehler; T. Nobis; H. Richter; I. Engels; K. Biehler; U. Frank

doi:10.1055/s-0032-1331754

Drug Research, Table of Contents

Drug Res (Stuttg) 2013; 63(02): 65-68
DOI: 10.1055/s-0032-1331754

Original Article

Broad Spectrum Antibacterial Activity of a Mixture of Isothiocyanates from Nasturtium (Tropaeoli majoris herba) and Horseradish (Armoraciae rusticanae radix)

A. Conrad

¹Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany

,

D. Biehler

¹Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany

,

T. Nobis

¹Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany

,

H. Richter

¹Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany

,

I. Engels

¹Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany

,

K. Biehler

¹Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany

,

U. Frank

¹Institut für Umweltmedizin und Krankenhaushygiene (Department of Environmental Health Sciences), University Medical Center Freiburg, Freiburg, Germany

› Author Affiliations

Abstract

Isothiocyanates have been reported to exert antimicrobial activity. These compounds are found in a licensed native preparation of nasturtium (Tropaeoli majoris herba) and horseradish (Armoraciae rusticanae radix) which is used for treatment of upper respiratory and urinary tract infections. The aim of our investigation was to assess the antimicrobial activity of a mixture of the contained benzyl-, allyl-, and phenylethyl- isothiocyanates against clinically important bacterial and fungal pathogens including antimicrobial resistant isolates. Susceptibility testing was performed by agar-dilution technique. Isothiocyanates were mixed in proportions identical to the licensed drug. Minimum inhibitory- and minimum bactericidal concentrations were assessed. The Minimum inhibitory concentration₉₀ was defined as the concentration which inhibited 90% of the microbial species tested. H. influenzae, M. catarrhalis, S. marcescens, P. vulgaris, and Candida spp. were found to be highly susceptible, with minimum inhibitory concentration₉₀ -values ranging between ≤0.0005% and 0.004% (v/v) of total ITC. Intermediate susceptibilities were observed for S. aureus, S. pyogenes, S. pneumoniae, K. pneumoniae, E. coli and P. aeruginosa, with Minimum inhibitory concentration₉₀ -values ranging between 0.004% and 0.125% (v/v), but with elevated Minimum bactericidal concentrations₉₀-values (2–7 dilution steps above Minimum inhibitory concentration₉₀). Low susceptibilities were determined for viridans streptococci and enterococci. Interestingly, both resistant and non-resistant bacteria were similarly susceptible to the test preparation.

Key words

isothiocyanate - Tropaeoli majoris herba - Armoraciae rusticanae radix - antimicrobial activity

Full Text

References

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