Bile acids (BA) may function as signaling molecules in a number of peripheral target
tissues. It has already been shown that BA confer resistance to diet-induced obesity
(DIO) in brown adipose tissue of C57BL/6J (B6) mice by inducing thermogenesis. In
our study, we aimed to assess the relevance of brown adipocytes within white adipose
tissue depots in this context.
B6 and 129Sv/Ev mice were assigned to one of four different diet groups: low fat (LFD)
or high fat (HFD) diet either with or without cholic acid supplementation. Energy
assimilation, body mass development as well as body composition were determined. Inguinal
white adipose tissue (iWAT) was screened for brite adipocyte recruitment by preparing
histological sections as well as conducting quantitative PCR measurements of brown
adipocyte markers. Moreover, plasma BA concentration was measured by HPLC-MS/MS.
Both mouse strains displayed HFD-induced obesity. Cholic acid supplementation of LFD
did not alter the body mass trajectory. As expected, feeding the HFD supplemented
with cholic acid protected B6 mice from DIO. Surprisingly, cholic acid supplementation
did not attenuate DIO in HFD fed 129Sv/Ev mice. Since energy assimilation was not
affected by dietary BA, cholic acid must exert its protective effect in B6 mice by
increasing energy expenditure.
This suggestion was underlined by iWAT analyses: Cholic acid supplementation caused
brite adipocyte recruitment exclusively in HFD fed B6 mice, indicated by the morphological
appearance of multilocular cells as well as increased brown adipocyte marker expression
levels.
Moreover, plasma BA concentration was considerably increased in HFD containing cholic
acid fed B6, but not 129Sv/Ev mice.
We identified 129Sv/Ev as a mouse strain resistant to the effects of dietary cholic
acid supplementation. In future, this animal model may serve to describe the underlying
mechanisms of resistance to diet-induced obesity conferred by bile acids.