Planta Med 2013; 79(02): 116-122
DOI: 10.1055/s-0032-1328062
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Protective Effect of Paeonia anomala Extracts and Constituents against tert-Butylhydroperoxide-Induced Oxidative Stress in HepG2 Cells

Sarangerel Oidovsambuu
1   Functional Food Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do, Republic of Korea
2   University of Science and Technology, Daejeon, Republic of Korea
Chul Young Kim
3   College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do, Republic of Korea
Kyungsu Kang
1   Functional Food Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do, Republic of Korea
Batsuren Dulamjav
4   Institute of Chemistry and Chemical Technology, Ulaanbaatar, Mongolia
Tunsag Jigjidsuren
4   Institute of Chemistry and Chemical Technology, Ulaanbaatar, Mongolia
Chu Won Nho
1   Functional Food Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do, Republic of Korea
› Author Affiliations
Further Information

Publication History

received 25 June 2012
revised 15 November 2012

accepted 15 November 2012

Publication Date:
24 January 2013 (online)


The fruit and root parts of Paeonia anomala L. are used for the treatment of many kinds of disorders in Mongolian traditional medicine. The protective effect of a fruit extract from P. anomala against tert-butylhydroperoxide-induced cell damage was evaluated in human hepatoma HepG2 cells and compared to that of a root extract from P. anomala on the basis of cell viability, generation of intracellular reactive oxygen species, cellular total glutathione concentration, and anti-genotoxicity. The fruit extract of P. anomala showed excellent protection against the oxidative stress when compared to the root extract, through free radical scavenging, enhancing cellular glutathione concentration, and inhibiting DNA damage. Chemical constituents in the fruit extract of P. anomala were investigated and two novel compounds, 2-hydroxy-6-methoxy-4-O-(6′-O-α-L-arabinofuranosyl-β-D-glucopyranosyl)acetophenone (1) and 3,3′-di-O-methyl-4-O-(3′′-O-galloyl-β-D-glucopyranosyl)ellagic acid (2), along with 18 other known compounds were identified. Compound 2 showed better cytoprotection against tert-butylhydroperoxide than compound 1. Among other compounds isolated from the fruit extract, ellagic acid, methyl gallate, ethyl gallate, fischeroside B, and quercetin derivatives showed potent protective effects against tert-butylhydroperoxide-induced oxidative stress via inhibiting reactive oxygen species generation and increasing total glutathione levels in HepG2 cells.

Supporting Information

  • References

  • 1 Ligaa U, Davaasuren B, Ninjil N. Medicinal plants of Mongolia used in Western and Eastern medicine. Moscow: Rosselhozakademii; 2009: 208
  • 2 Li Xian LSH, Pu JX, Huang SX, Sun HD. Chemical constituents from Paeonia anomala subsp. veitchii (Paeoniaceae). Acta Bot Yunn 2007; 29: 259-262
  • 3 Zapesochnaya GG, Kurkin VA, Avdeeva EV, Popov DM, Kolpakova MV. A chemical study of the roots of Paeonia anomala . Khimiya Prirodnykh Soedinenii 1992; 1: 55-59
  • 4 Volodya T, Tserenbaljir D, Lkhamjav T. Mongolian medicinal plants. Ulaanbaatar: Admon; 2008: 334-336
  • 5 Kohen R, Nyska A. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol 2002; 30: 620-650
  • 6 Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39: 44-84
  • 7 Alia M, Ramos S, Mateos R, Granado-Serrano AB, Bravo L, Goya L. Quercetin protects human hepatoma HepG2 against oxidative stress induced by tert-butyl hydroperoxide. Toxicol Appl Pharmacol 2006; 212: 110-118
  • 8 Taffe BG, Takahashi N, Kensler TW, Mason RP. Generation of free radicals from organic hydroperoxide tumor promoters in isolated mouse keratinocytes. Formation of alkyl and alkoxyl radicals from tert-butyl hydroperoxide and cumene hydroperoxide. J Biol Chem 1987; 262: 12143-12149
  • 9 Alia M, Ramos S, Mateos R, Bravo L, Goya L. Response of the antioxidant defense system to tert-butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2). J Biochem Mol Toxicol 2005; 19: 119-128
  • 10 Pan MH, Ho CT. Chemopreventive effects of natural dietary compounds on cancer development. Chem Soc Rev 2008; 37: 2558-2574
  • 11 Duan WJ, Yang JY, Chen LX, Zhang LJ, Jiang ZH, Cai XD, Zhang X, Qiu F. Monoterpenes from Paeonia albiflora and their inhibitory activity on nitric oxide production by lipopolysaccharide-activated microglia. J Nat Prod 2009; 72: 1579-1584
  • 12 Kang K, Jho EH, Lee HJ, Oidovsambuu S, Yun JH, Kim CY, Yoo JH, Kim YJ, Kim JH, Ahn SY, Nho CW. Youngia denticulata protects against oxidative damage induced by tert-butylhydroperoxide in HepG2 cells. J Med Food 2011; 14: 1198-1207
  • 13 Pfundstein B, El Desouky SK, Hull WE, Haubner R, Erben G, Owen RW. Polyphenolic compounds in the fruits of Egyptian medicinal plants (Terminalia bellerica, Terminalia chebula and Terminalia horrida): characterization, quantitation and determination of antioxidant capacities. Phytochemistry 2010; 71: 1132-1148
  • 14 Washida K, Yamagaki T, Iwashita T, Nomoto K. Two new galloylated monoterpene glycosides, 4-O-galloylalbiflorin and 4′-O-galloylpaeoniflorin, from the roots of Paeonia lactiflora (Paeoniae radix) grown and processed in Nara prefecture, Japan. Chem Pharm Bull (Tokyo) 2009; 57: 1150-1152
  • 15 Kurashima K, Fujii M, Ida Y, Akita H. Enzymatic β-glycosidation of primary alcohols. J Mol Catal B Enzym 2003; 26: 87-98
  • 16 Lopez G, Nugier-Chauvin C, Remond C, OʼDonohue M. Investigation of the specificity of an α-L-arabinofuranosidase using C-2 and C-5 modified α-L-arabinofuranosides. Carbohydr Res 2007; 342: 2202-2211
  • 17 He CN, Peng Y, Zhang YC, Xu LJ, Gu J, Xiao PG. Phytochemical and biological studies of Paeoniaceae. Chem Biodivers 2010; 7: 805-838
  • 18 Shon YH, Nam KS. Protective effect of moutan cortex extract on acetaminophen-induced hepatotoxicity in mice. J Ethnopharmacol 2004; 90: 415-419
  • 19 Su J, Zhang P, Zhang JJ, Qi XM, Wu YG, Shen JJ. Effects of total glucosides of paeony on oxidative stress in the kidney from diabetic rats. Phytomedicine 2010; 17: 254-260
  • 20 Wang H, Wei W, Wang NP, Wu CY, Yan SX, Yue L, Zhang LL, Xu SY. Effects of total glucosides of peony on immunological hepatic fibrosis in rats. World J Gastroenterol 2005; 11: 2124-2129
  • 21 Wu YG, Ren KJ, Liang C, Yuan LM, Qi XM, Dong J, Shen JJ, Lin SY. Renoprotective effect of total glucosides of paeony (TGP) and its mechanism in experimental diabetes. J Pharmacol Sci 2009; 109: 78-87
  • 22 Hayes JD, Mclellan LI. Glutathione and glutathione-dependent enzymes represent a co-ordinately regulated defence against oxidative stress. Free Radic Res 1999; 31: 273-300
  • 23 Ramos AA, Lima CF, Pereira ML, Fernandes-Ferreira M, Pereira-Wilson C. Antigenotoxic effects of quercetin, rutin and ursolic acid on HepG2 cells: evaluation by the comet assay. Toxicol Lett 2008; 177: 66-73
  • 24 Fairbairn DW, Olive PL, Oneill KL. The comet assay – a comprehensive review. Mutat Res 1995; 339: 37-59