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Endoscopy 2013; 45(02): 79-85
DOI: 10.1055/s-0032-1325762
Original article
© Georg Thieme Verlag KG Stuttgart · New York

In vivo molecular imaging of gastric cancer by targeting MG7 antigen with confocal laser endomicroscopy

Z. Li
1   Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan, China
2   Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Qilu Hospital, Shandong University, Jinan, China
,
X. L. Zuo
1   Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan, China
,
C. Q. Li
1   Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan, China
,
C. J. Zhou
3   Department of Pathology, the Second Affiliated Hospital, Shandong University, Jinan, China
,
J. Liu
1   Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan, China
,
M. Goetz
4   I. Medical Clinic, University of Mainz, Mainz, Germany
6   Leiter der Interdisziplinären Endoskopie, Innere Medizin 1, Universitätsklinikum Tübingen, Otfried-Müller-Str. 10, D-72076 Tübingen
,
R. Kiesslich
4   I. Medical Clinic, University of Mainz, Mainz, Germany
,
K. C. Wu
5   State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, China
,
D. M. Fan
5   State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, China
,
Y. Q. Li
1   Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan, China
› Author Affiliations
Further Information

Publication History

submitted 23 February 2012

accepted after revision 03 August 2012

Publication Date:
30 January 2013 (online)

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Commentaire de travail de Z. Li et al., pp.79Endoscopy 2013; 45(02): 232-232
DOI: 10.1055/s-0032-1326334

Background and study aims: In vivo molecular imaging represents a powerful tool for the immediate diagnosis of gastric cancer. In this study, the monoclonal antibody MG7, which is a specific molecular marker against gastric cancer, was labeled with fluorescent agents to enable in vivo real-time imaging by confocal laser endomicroscopy (CLE).

Patients and methods: In vivo molecular imaging was performed in tumor-bearing mice from two kinds of human gastric cancer cell lines. Xenograft tumors were visualized in vivo first with a whole-body fluorescent imaging device and then by CLE using fluorescently labeled MG7 antibody. Gastric cancerous tissue and noncancerous mucosa from human biopsies or surgical specimens were also examined ex vivo by CLE.

Results: Intravital imaging of xenograft tumors revealed a specific cellular signal, whereas no specific signal was observed in control tissue or in mice injected with irrelevant antibodies. An ex vivo experiment on human specimens using a rigid confocal probe showed positive fluorescent staining in 22 /23 samples diagnosed as gastric cancer and weak signals in 5 /23 noncancerous tissue samples. CLE evaluation correlated well with immunohistochemical findings.

Conclusions: Screening tumors in vivo by CLE may help to detect MG7-Ag-positive tissues, decrease the sampling error by screening the large tumor surface not routinely screened by biopsy or conventional immunohistochemistry, and facilitate early detection of gastric carcinoma.

 

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