Profiling of cereal-derived alkylresorcinols with antigungal activity against Fusarium oxysporum

Alkylresorcinols are non-isoprenoid phenolic lipids present in several commercial plants of Poaceae family. In vitro studies on alkylresorcinols have shown to possess antimutagenic, antimicrobial, cardioprotective activities among others. As part of our research on antifungal agents, a chemical characterization focused on alkylresorcinol profiling and quantification by HPLC-ESI-MS and Fast Blue RR-based colorimetric method, respectively, were performed on alkylresorcinol-enriched extracts (AEE) from wheat, corn, oats, barley and wheat bran flours. Several alkylresorcinol were identified in each product, whose common structural feature was the alkenyl side chain. Wheat bran was found to have the highest alkylresorcinol content followed by barley, wheat, oats, and maize flours. In addition, in vitro antifungal assay was performed on each AEE against Fusarium oxysporum. All AEE exhibited dose dependent antifungal activity at different levels. All data were correlated by principal component analysis (PCA). A composition-activity relationship was observed.