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DOI: 10.1055/s-0032-1321353
Micropropagation and identification of O-palmitoyl-3-O-β-D-glucoside of β-sitosterol in the medicinal species Lopezia Racemosa
Lopezia racemosa Cav is a traditional medicinal plant with anti-inflammatory and anti-cancer properties. Among the secondary compounds of interest is O-palmitoyl-3-O-β-D-glucoside of β-sitosterol (LR1) that seems to participate in the medicinal effects of this species. The aim of this work was to obtain an efficient micropropagation system of L. racemosa to compare the active metabolites contents between in vitro and field-grown plants. Cultures were initiated from nodal segments of in vitro germinated seedling inoculated onto Murashige and Skoog (MS) medium supplemented with BA individually or in combination with NAA. Optimum culture conditions for shoot organogenesis were determined. Multiple shoots were generated via indirect organogenesis on medium with either BA 0.5mg/L or combined with NAA 2.5mg/L. The elongated shoots were successfully acclimated to greenhouse conditions. Thin layer chromatography (TLC) and proton nuclear magnetic resonance spectroscopy (1H-NMR) analysis showed the presence of LR1 in callus, regenerated shoots and the control plants. Therefore, the protocol also provides an effective means for the in vitro conservation of L. racemosa that produce pharmaceutically interesting anti-inflammatory and anti-cancer metabolites.