Planta Med 2012; 78 - PJ111
DOI: 10.1055/s-0032-1321271

Production of quinonemethide triterpenes by Peritassa laevigata cell culture

E Souza Pina 1, A Aparecida Lopes 2, J da Silva Coppede 1, BW Bertoni 1, S de Castro França 1, AM Soares Pereira 1
  • 1University of Ribeirao Preto, 14100–000
  • 2Faculty of Pharmaceutical Science of Ribeirao Preto, USP, 14040–903 Ribeirao Preto, SP, Brazil;.

Peritassa laevigata (Hoffmanns. Ex. Link.) A. C. S., is a plant from the Celastraceae family native to the Cerrado biome. Quinonemethide triterpenes derived from the friedo-nor-oleanane skeleton are among a number of secondary metabolites produced by this species. Reports on the pharmacological activity of the quinonemethides evidenced their antitumor efficacy. The aim of this work was to investigate the production of quinonemethide-type triterpenoids in root of P. laevigata cultivated in vitro. Peritassa laevigata roots growing easily and the presence of IBA regulators had positive effect on the cell culture. The dicloromethane extract from the roots of Peritassa laevigata was analyzed by HPLC. Separation carried out on a C-18 analytical column (25×4.6mm 5µm), mobile phase isocratic MeOH:H2O (85:15), 1.0 mL/min and UV absorption was determined at 254 and 420nm simultaneously using the PAD. Compound 1 was detected on the extract, showing that the terpene biosynthetic ability has been maintained in the cell culture. These studies will be used to further investigate quinonemethide triterpenes biosynthesis pathways.