Maitenin in cell line of Maytenus ilicifolia (Celastraceae) Reissek front different sources of carbon

The species Maytenus ilicifolia ex Reissek which produces triterpenes quinonametideos exhibit cytotoxic activity, however these compounds are stored only in the bark of the roots of the plant and reduced concentrations it difficult to use of these active compounds on an industrial scale. The objective of the present work is to evaluate the production of maitenin in cell suspension cultures of the species M. ilicifolia cultivated in different carbon sources. Cell lines maintained for thirty-five days in culture medium. This cell was transferred (2g) to 60mL of MS medium + 30g/L sucrose + 1mg/L 2,4-D and 0,5mg/L kinetin. The cells were maintained in growth room with controlled temperature and photoperiod, next the cells were filtered and dried in circulating air oven. The drug plant was subjected to maceration with dichloromethane in a ultrasonic bath for 20 minutes three times. The extract generated was analyzed using HPLC C-18 column, phase mobile methanol: water 85:15 + 0.1% formic acid at a flow rate of 1mL/min for 25 minutes. The chromatograms generated showed that difference was found in the productivity of maitenin and cells cultivated in medium culture containing sucrose showed higher contents of triterpene production when compared to the same asset obtained from the cultivation on glucose.