Rapid on-line dereplication by HPTLC-MS interface in orchid extracts

Previous phytochemical analyses of crude extracts of Vanda coerulea Griff ex. Lindl (Orchidaceae) have led to the identification of stem-specific metabolites. The three most concentrated stilbenoids (imbricatin, methoxycoelonin and gigantol) displayed complementary biological activities in slowing down the skin cell senescence process and can thus be considered as biomarkers of this orchid species [1]. V. coerulea stem extract is now used in cosmetics for its anti skin ageing effect.

In search for new bioactive compounds in orchids, other species from the same Vandeae tribe were explored. In order to accelerate the isolation of new molecules, a dereplication approach using HPTLC-MS interface was developed to quickly identify the three markers previously mentioned and to priority isolate other metabolites.

Using this technique, the stem-specific biomarkers of V. coerulea were proven to be also present in other genera, along with other phenanthrene derivatives. This performing tool not only permits to provide a quick dereplication approach, but also to help us determine if these bioactive compounds could be considered as taxonomic markers of the Vandeae tribe.