Centrifugal partition chromatography for the isolation of tropical orchid constituents

V Cakova; A Urbain; A Sester; P André; F Bonté; A Lobstein

doi:10.1055/s-0032-1321265

Planta Medica, Table of Contents

Centrifugal partition chromatography for the isolation of tropical orchid constituents

V Cakova ¹^,², A Urbain ¹, A Sester ¹, P André ², F Bonté ², A Lobstein ¹

¹Pharmacognosy and Bioactive Natural Products, UMR 7200, University of Strasbourg, BP 60024, 67401 Illkirch, France
²LVMH Recherche, 185 avenue de Verdun, 45800 Saint-Jean de Braye, France

Abstract

Orchidaceae is one of the largest botanical families and some species have been used as ingredients of traditional preparations with beneficial properties. Despite the high popularity of these plants, there are really few studies concerning their chemical composition. In the aim to acquire more basic knowledge about metabolites from different tropical orchids, several species were selected. The present study deals with the rapid isolation of orchid constituents by centrifugal partition chromatography (CPC). Dried aerial parts were extracted with CH₂Cl₂, and the obtained extract was fractionated by CPC using the solvent system n-heptane-ethyl acetate-methanol-0,1% formic acid. Different proportions were tested to find convenient solvent conditions for the separation of potential chemotaxonomic markers.