Rapid scaleup of high performance countercurrent chromatography from bench to kilogram

GH Harris; C De Amicis; NA Edwards; MB Giles; P Hewitson; L Janaway; S Ignatova

doi:10.1055/s-0032-1321264

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Planta Med 2012; 78 - PJ104
DOI: 10.1055/s-0032-1321264

Rapid scaleup of high performance countercurrent chromatography from bench to kilogram

GH Harris ¹, C De Amicis ², NA Edwards ³, MB Giles ³, P Hewitson ⁴, L Janaway ³, S Ignatova ⁴

¹Dynamic Extractions, Inc., 11 Deer Park Drive, Suite 200, Monmouth Junction, New Jersey 08852, USA
²Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, IN 46268, USA
³Dynamic Extractions, Ltd., 890 Plymouth Road, Slough, Berkshire, UK
⁴Advanced Bioprocessing Centre, Brunel University, Uxbridge, Middlesex, UB8 3PH, UK

Congress Abstract

Countercurrent chromatography (CCC) has found broad application for natural product purification at the discovery scale but one limitation to the practical use of the technique has been rapid and predictable scaleup. This limitation has been overcome using HPCCC (HPCCC) instrumentation. As an example, method development and the kilogram scale purification of two semi-synthetic spinosyns, spinetoram-J (1) and -L (2), at the kilogram scale will be described.