Isolation and identification of flavonoids from Annona mucosa

Annona mucosa, a typical tropical fruit tree, is widely distributed in Brazil. It is used in folk medicine as a therapeutic agent in the treatment of tumors and its phytochemical study revealed it as great source of secondary metabolites such as annonaceous acetogenins, alkaloids and lignans with several biological activities. The present study aimed to perform the phytochemical study of polar extracts of this plant in order to contribute to its phytochemical profile and find new polar compounds that can also be related to those biological activities. The phytochemical study of the ethyl acetate fraction of the partition of ethanolic extract of leaves of A. mucosa allowed the isolation of four flavonoids identified as isoquercetin (1), hyperin (2), rutin (3) and astragalin (4). These flavonoids were isolated by the first time from this plant. The isolation procedures and identification data (NMR and LC-MS) will be presented.