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DOI: 10.1055/s-0032-1321230
Mexican propolis research: HPLC determination of the major flavonoids
Propolis is a natural resinous substance collected by bees from aerial parts of the plants, buds or exudates. The chemical composition of the plant source its related with the composition of bee glue. Combined with the knowledge of the active principles, it gives clues to standardization and quality control procedures, allowing the specification of propolis types that have different chemical composition. Therefore, a validated HPLC method for quantifying acacetin (1), 4,7-dimethyl naringenin (2), and 4′,7-dimethyl apigenin (3), main flavonoids present in the Mexican propolis, was developed. For each compound a linear response was evaluated within the range of 0.5–2.0mg/mL for 1, 0.25–1.25mg/mL for 2, and 0.05–0.5mg/mL for 3.