Planta Med 2012; 78 - PJ58
DOI: 10.1055/s-0032-1321218

Qualitative and quantitative determination of bioactive marker compounds from Gastrodia elata by HPLC-DAD-MS

JY Kwon 1, N Kim 1, DH Lee 2, AR Han 3, JW Lee 2, EK Seo 3, JH Lee 4, D Lee 1
  • 1School of Life Sciences and Biotechnology, Korea University, Seoul 136–713, Korea
  • 2Department of Statistics, Korea University, Seoul 136–701
  • 3College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University, Seoul 120–750
  • 4College of Oriental Medicine, Dongguk University, Gyeongju 780–714

Qualitative and quantitative determination of four bioactive compounds including gastrodin, gastrodigenin, p-hydroxybenzaldehyde, bis(4-hydroxybenzyl)ether isolated from Gastrodia elata has been developed using high performance liquid chromatography-diode array detector coupled with electrospray ionization/mass spectrometry (HPLC-DAD-MS). The analysis of four standard compounds showed good linearity, intra- and inter-day precisions, and accuracy of them. In addition, metabolite profiling of 23 different Gastrodia elata extracts was carried out to identify their origins. Total analyzed metabolites were applied to multivariate statistical analysis including principal component analysis and hierarchical clustering analysis for pattern analysis.