HPLC-MS3 identifying profiles of Astragalus membranaceus var. mongholicus for diabetic nephropathy and peripheral neuropathy

Y Liu; S Sun; H Zhang; Y Zhang; J Shen

doi:10.1055/s-0032-1321191

Planta Medica, Inhaltsverzeichnis

HPLC-MS3 identifying profiles of Astragalus membranaceus var. mongholicus for diabetic nephropathy and peripheral neuropathy

Y Liu ¹, S Sun ¹, H Zhang ¹, Y Zhang ², J Shen ²

¹Institute of Botany (Nanjing Botanical Garden Mem. Sun Yat-Sen), Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu 210014, China
²Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu 210029, China

Abstract

The dried roots of Astragalus membranaceus or A . membranaceus (Fisch.) Bge var. mongholicus, Radix Astragali, was prescribed more than 80% in compound traditional Chinese medicines for diabetes mellitus. Current research found Radix Astragali demonstrates pharmacological effect on diabetic complications such as diabetic nephropathy (DN) and diabetic peripheral neuropathy (DPN) as well as diabetes mellitus. We carry on the study on bioactive fraction and compounds of Radix Astragali for DN and DPN. The bioactive fraction was identified by LC/MS³ on the basis of separated active compounds, besides astragaloside IV (ASI) was focus on in our long term study. 16 peaks in the HPLC spectrum were determined by analysis their ESI-MS³ spectra and retention time with isolated compounds and referring to literatures. Astragalosides and flavonoides are the main constituents in bioactive fractions.