Simultaneous determination of 12 bioactive compounds in 'Jaeumganghwa-tang' by HPLC-DAD
Jaeumganghwatang is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus. A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for simultaneous determination of 13 major compounds, decursin, decursinol, nodakenin, paeoniflorin, 5-HMF, hesperidin, naringin, berberine, mangiferin, glycyrrhizin, catalpol and aucubin in Jaeumganghwa-tang. The separation use Shishedo C18 column and detection wavelength was set at 205, 250, 280 and 300nm. The developed analyses had good linearity (R2>0.9997). The range of limit of detection (LOD) and limit of quantification (LOQ) were observed from 0.08 to 1.05 and from 0.3 to 3.17. The intra- and inter-day test was indicated less than 3%. The recoveries were between 95.22 - 101.01%. This HPLC-DAD method is successfully applied for simultaneous determination of 12 major compounds in Jaeumganghwa-tang samples.