Planta Med 2012; 78 - PJ6
DOI: 10.1055/s-0032-1321166

Validation of HPLC assay for the determination of oxyprenylated anthraquinones produced by Rhamnus L. species

M Locatelli 1, G Carlucci 1, F Epifano 1, D Kremer 2, S Genovese 1
  • 1Dipartimento di Scienze del Farmaco, Università degli Studi ''G. d'Annunzio'' Chieti-Pescara, Via dei Vestini 31, 66100 Chieti (CH), Italy
  • 2Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, Croatia

Rhamnus L. spp. (Rhamnaceae) is known to contain active anthraquinones secondary metabolites but the presence of oxyprenylated ones is still not reported. As continuation of our studies [1, 2], a new method [3] was validated to quantify these analytes in plant extracts after extraction with n-hexane and methanol using a ODS column, water and methanol (1% formic acid, v/v) as mobile phase at 0.7 mL min-1 in gradient elution. Quantitative analyses, at 435nm, revealed LOQ of 0.5µM and linearity up to 125µM. Precision values ranged from 0.2% to 12.9% while trueness from 12.2% to 12.7%.

References

[1] Locatelli M. Current Drug Targets (2011) 12:366–380. [2] Kremer D. et al., Food Chemistry (2012) 131:1174–1180. [3] Locatelli M. et al., Journal of Chromatography A (2012) 1225:113–120.