Planta Med 2012; 78 - PI418
DOI: 10.1055/s-0032-1321105

Isolation of 1, 2, 3, 4, 11, 12-hexadehydrositost-5-en-3β-ol with mild hepatoprotective effect from Landolphia owariensis P. Beauv.

PO Osadebe 1, TJN Okonkwo 2
  • 1Pharmaceutical and Medicinal Chemistry Department, University of Nigeria, Nsukka, Enugu State, Nigeria
  • 2Pharmaceutical and Medicinal Chemistry Department, University of Port Harcourt, Choba, Port Harcourt, Rivers State, Nigeria

1, 2, 3, 4, 11, 12-hexadehydrositost-5-en-3β-ol (hexadehydro-3β-sitosterol) has been isolated from the n-hexane extract of Landolphia owariensis P. Beauv. stringy seed pulp. The chemical structure was confirmed by GC-MS and H-nmr spectroscopy. This is the first report of isolation of this compound from the plant. The isolated compound marginally reversed thioacetamide (800mg/kg, i.p.) induced elevation of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP), with respect to normal control and silimarin (300mg/kg) positive control rats; as well as reduced the hepatotoxin induced rise of serum albumin (ALB), total protein (TP), total bilirubin (TB), direct and indirect bilirubin (DB and IDB, respectively). Hepato-histology of the treated and control animals, further confirmed the mild hepatoprotective effect of 1, 2, 3, 4, 11, 12-hexadehydrositost-5-en-3β-ol. Taken together, 1, 2, 3, 4, 11, 12-hexadehydrositost-5-en-3β-ol is minor contributor to the confirmed hepatoprotective properties of Landolphia owariensis P. Beauv stringy seed pulp; and is suspected to possess cholesterol lowering effect in animal models.

1, 2, 3, 4, 11, 12-Hexadehydrositost-5-en-3β-ol(Hexadehydro-3β-sitosterol)