Phenolic compounds as possible targets for quality control of HPLC fingerprint

LL Saldanha; PMP Vareda; JR Bosqueiro; VF Ximenes; W Vilegas; AL Dokkedal

doi:10.1055/s-0032-1321081

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Planta Med 2012; 78 - PI394
DOI: 10.1055/s-0032-1321081

Phenolic compounds as possible targets for quality control of HPLC fingerprint

LL Saldanha ¹, PMP Vareda ¹, JR Bosqueiro ², VF Ximenes ², W Vilegas ³, AL Dokkedal ²

¹Institute of Bioscience, UNESP – Univ Estadual Paulista, Botucatu, SP, Brazil
²Faculty of Sciences, UNESP – Univ Estadual Paulista, Bauru, SP, Brazil
³Chemistry Institute, UNESP – Univ Estadual Paulista, Araraquara, SP, Brazil

Congress Abstract

Hydroalcoholic extract of Myrcia bella Cambess. leaves was evaluated by HPCL-ESI-MS/MS, ESI-MS/MSⁿ, HPLC-PDA and streptozotocin-induced diabetes model. The fingerprint revealed as major flavonoids 6 and 10, with known inhibition activity on diabetes related enzymes. The extract presents hypolipidemic and hypoglycemic activity. Therefore 6 and 10 could be active principles and possible targets for the quality control of M. bella extract.

A HPLC-PDA chromatogram at 254nm. Peak: 1 cafeic acid, 2 quinic acid, 3 gallic acid, 4 myricetin-O-hexoside, 5 myricetin rhamnoside-galloyl, 6 myricetin-O-deoxyhexoside, 7 myricetin-7-O-rhamnoside, 8 quercetin-O-pentoside, 9 quercetin-O-pentoside, 10 quercetin-O-pentoside, 11 isorhamnetin-O-pentoside, 12 quercetin-O-pentoside-galloyl, 13 quercetin. B Flavonoids quantification based on quercetin calibration curves (R² >0.9995) at 360nm. *The values are means ±SD, n=9. C Metabolic parameters of normal and diabetic mice treated with extract and saline (p<0,005), n=8.