Planta Med 2012; 78 - PI378
DOI: 10.1055/s-0032-1321065

UPLC-HRMS based profiling of Micromelum falcatum extracts and isolation of coumarin derivatives

E Danika 1, E Kouloura 1, K Sothea 2, M Halabalaki 1, AL Skaltsounis 1
  • 1Laboratory of Pharmacognosy & Natural Products Chemistry, School of Pharmacy, Panepistimioupoli, Zografou, 15771, Athens, Greece
  • 2Joint Laboratory of Phytochemistry, Faculty of Pharmacy, University of Health Sciences 73, Bd Monivong, Phnom Penh, Cambodia

Micromelum falcatum (Rutaceae) is a small tree growing in West Asian regions1 used against cold and rheumatoid arthritis according to the traditional medicine of China2. The leaves of the plant, collected in Cambodia, were extracted following two extraction protocols and six different extracts were obtained. The analytical profiling of all extracts was performed using HPLC-DAD and LC-MS techniques leading to the detection of coumarin derivatives in the majority of the extracts. In parallel, a UPLC-ESI(+)-HRMS method using an Orbitrap analyzer was developed for the detection and characterization of coumarin derivatives in all extracts. All the compounds of interest were further isolated with semi-Preparative HPLC leading to the identification of seven coumarins among them three new natural products. The structure elucidation of all isolated compounds was performed via NMR and HRMS spectrometry and representative compounds have been evaluated for their anti-inflammatory activity.

1Zhang Dianxiang, Thomas G. Hartley, Micromelum Blume Bijdr., Flora of China, 2008, 11, 79–80 2Xi-long Zheng, Fu-wu Xing, Ethnobotanical study on medicinal plants around Mt.Yinggeling, Hainan Island, China, Journal of Ethnopharmacology 124, 2009, 197–210.