Bioassay-guided isolation of anti-Leishmania major compounds from Micromelum minutum

A Sakunpak; K Matsunami; H Otsuka; P Panichayupakaranant

doi:10.1055/s-0032-1321018

Planta Medica, Inhaltsverzeichnis

Bioassay-guided isolation of anti-Leishmania major compounds from Micromelum minutum

A Sakunpak ¹, K Matsunami ², H Otsuka ², P Panichayupakaranant ¹^,³

¹Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand
²Department of Pharmacognosy, Graduate School of Biomedical Sciences, Hiroshima University; 1–2-3 Kasumi, Minamiku, Hiroshima 734–8553, Japan
³Phytomedicine and Pharmaceutical Biotechnology Research Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand

Abstract

Anti-Leishmania major assay-guided isolation of Micromelum minutum leaves gave two new monoterpene coumarins, named minutin A and minutin B, together with four known coumarins, 8,4′′-dihydroxy-3′′,4′′-dihydrocapnolactone-2′,3′diol, 8-hydroxyisocapnolactone-2′,3′- diol, 8-hydroxy-3′′,4′′-dihydrocapnolactone-2′,3′-diol, and clauslactone E. Minutin A, minutin B, 8-hydroxyisocapnolactone-2′,3′-diol and clauslactone E showed satisfactory anti-L. major activity with IC₅₀ of 26.2, 20.2, 12.1, and 9.8µM, respectively, while 8,4′′-dihydroxy-3′′,4′′-dihydrocapnolactone-2′,3′-diol and 8-hydroxy-3′′,4′′-dihydrocapnolactone-2′,3′-diol were not active.