Planta Med 2012; 78 - PI314
DOI: 10.1055/s-0032-1321001

Anticancer activity of resveratrol analogs from Eugenia rigida DC

MA Zaki 1, 2, V Samoylenko 1, S Khan 1, RM Abd slam 2, MH Hetta 2, U Shin 3, J Pelletier 3, LA Walker 1, I Muhammad 1
  • 1National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, Mississippi 38677, USA
  • 2Department of Pharmacognosy, School of Pharmacy, Beni-Suef University, Beni-Suef, Egypt
  • 3McIntyre Medical Sciences, McGill University, Montreal H3G 1Y6, Quebec, Canada

Two new natural resveratrol analogs, namely 3,5,3',4'-tetramethoxy-(trans)-piceatannol (1) and 3,5,3'.4'-tetramethoxy-(cis)-piceatannol (2), together with the known 3,5,4'-trimethoxy-(trans)-resveratrol (3) have been isolated from the leaves of Eugenia rigida. The structures of 1 and 2 were characterized using NMR and MS techniques. The trans (1) and cis- isomers (2) were evaluated for anti-cell proliferative activity against selected human cancer cell line and non-cancerous VERO cells. The cis- isomer showed anti-cell proliferative activity with IC50 values ranging between 5.3–16.6µM, while the trans- isomer (1) was inactive up to 33.3µM. Furthermore, the activity against intracellular reactive oxygen species (ROS) generation was also determined. The trans-isomer showed an inhibition of 50% in ROS generation at 33.3µM in PMA-induced HL-60 cells, while cis-isomer did not exhibit any effect. Finally, both the isomers were studied for the inhibition of protein biosynthesis in vitro in a translation system derived from the Krebs cells.