Planta Med 2012; 78 - PI267
DOI: 10.1055/s-0032-1320954

Phytochemical and biological studies of two synonymy species of Solanum: S. incanum and S. heteracanthum

M Jaovita Manase 1, AC Mitaine-Offer 1, D Pertuit 1, T Miyamoto 2, C Tanaka 2, S Delemasure 3, P Dutartre 3, JF Mirjolet 4, O Duchamp 4, MA Lacaille-Dubois 1
  • 1EA 4267 (FDE/UFC), UFR de Pharmacie, Université de Bourgogne, Dijon, France
  • 2Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
  • 3Cohiro, UFR de Médecine, Dijon, France
  • 4Oncodesign, Dijon, France

A new spirostanol saponin (1), with four known saponins, dioscin (2), protodioscin (3), methylprotodioscin (4), indioside D (5) and one known glycoalkaloid solamargine (6) were isolated from the two synonymous species, Solanum incanum and S. heteracanthum. 1 was established as (23S,25R)-spirost-5-en-3β,23-diol 3-O-{β-D-xylopyranosyl-(1→2)-O-α-L-rhamnopyranosyl-(1→4)-[O-α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside}, by using a combination of 1D and 2D NMR techniques including 1H, 13C, COSY, TOCSY, NOESY, HSQC and HMBC experiments and by mass spectrometry. 1, 3, 4 and 5 were evaluated for cytotoxicity and antioxidant activities against six cancer cell lines (HCT 116, HT-29, SW480, DU145, EMT6 and THP-1).