In vitro assay of ethanol extract from Syzygium cumini (L.) Skeels leaves against bacteria that cause dermatitis in pets

Dermatitis is an inflammation of deep slayers of skin, which could cause cell lesion generated for different agents. The increase of resistance against bacteria that causes dermatitis in pets have been worried veterinaries in Brazil. Thus, becomes necessary the discovery of new agents effective, as the rich mixture of compounds found in plant extracts. Here we intent to evaluate an ethanol extract of Syzygium cumini leaves at 0,2mg/ml EtOH, which was obtained from fresh leaves. The agar diffusion method was used and the bacteria tested were isolated from dermatitis of pets treated at UPIS Veterinary Hospital. Gram negative and Gram positive strains were used in vitro assays. After 24h, growing inhibition halo of 19mm was observed when we used 6mg/µl of extract against Staphylococcus intermedius and a 16mm halo was formed, using the same sample concentration, against Pseudomonas spp. This last strain, showed resistance against 16 different antimicrobial drugs. At the same concentration, this extract do not caused cell death in vitro assay using sheep blood. MIC of 2µg/µl of extract was determinate to S. intermedius and Pseudomonas spp. After toxicity tests, this extract could be used in vivo assays, aiming treatment of dermatitis in pets.